EVANOVO® is a live attenuated parasitic vaccine specially developed for in-ovo (IO) administration of chickens against coccidiosis. Combining in-ovo vaccination against coccidiosis with other commercial vaccines in a single injection can optimize and streamline hatchery vaccination processes without compromising the effectiveness of the co-administered vaccines. Therefore, the objective of this study was to assess the safety and immunogenicity of the associated administration of EVANOVO® and a Turkey herpesvirus double construct (HVT – ND – IBD) vaccine whe injected IO to 18-day-old chicken embryonated broiler eggs.