Can the PRRS ELISA results be different for serum or oral fluids?

Startseite Know-how Can the PRRS ELISA results be different for serum or oral fluids?

Can the PRRS ELISA results be different for serum or oral fluids?


Answered by: Enric Marco   I   Published on: August 25, 2017

The results obtained when performing an ELISA test from oral fluids or serum samples should be similar, provided that the type of test used is the same and the samples are from the same animal.

If the same type of test is not used (different manufacturers) there may be differences, but not because sample type was different, as the same could happen if both samples were serum. The proteins detected by the different ELISA test available in the market, are not always the same.

This fact affects the duration of the positivity detected that can differ between ELISAs. In any case, using the same type of test, what could happen is that the oral fluid samples give negative results faster than the serum samples (oral fluids are a dirtier sample than serum, and the presence of enzymes in it, could affect the proteins).

The PRRS saliva antibodies come from the bloodstream, so if it is the reverse outcome (positive results in oral fluids and negative result in serum) the sample should be repeated because it does not have much biological sense.

 

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