Evaluation of three ELISAs for detecting serum antibodies against Mycoplasma hyopneumoniae

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Evaluation of three ELISAs for detecting serum antibodies against Mycoplasma hyopneumoniae

Mycoplasma hyopneumoniae (Mhyo) is an important component of the so called Porcine Respiratory Disease Complex (PRDC). Mhyo infection in pigs is usually monitored through serology. In this study, we compared three ELISAs developed to detect antibodies to Mhyo in swine serum. The main objectives of this study were to differentiate serum antibody profiles of four groups of animals: non-challenged/vaccinated, challenged/vaccinated, challenged/non-vaccinated and non-challenged/non-vaccinated animals.

About this article

Appeared in

IPVS Congress, 2012

Authors

Martos, A.; Porquet, L.; Rebordosa, X.

Summary

The most similar kits were the CIVTEST® and the ELISA-C because both of them have a high sensitivity (Table 1). CIVTEST® has better performance differentiating quantitatively samples from vaccinated animals and samples from vaccinated/challenged animals. The high sensitivity of the ELISA-C when detecting vaccination seems to make this test have more problems when distinguishing between samples from vaccinated animals and samples from vaccinated/challenged animals.

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