Improving oedema disease diagnosis in pigs by detecting the vt2e toxin gen in oral fluid by qPCR

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Improving oedema disease diagnosis in pigs by detecting the vt2e toxin gen in oral fluid by qPCR

Oedema disease caused by verotoxigenic strains of Escherichia coli (VTEC) is often diagnosed by bacterial culture from intestinal contents. This method is time consuming and requires additional tests to ensure that the isolate has the potential to produce the toxin. Here we report on the use of oral fluid (OF) and Real Time PCR (qPCR) as alternative methods in terms of sampling, representativeness and cost. 

Informazioni sull'articolo

Pubblicato in

European Symposium of Porcine Health Management, 2018

Data

10 lug 2018

Autori

Valls, L.; Sánchez, A.; Maldonado, J.

Riepilogo

Results from this study demonstrate that the OF along with the qPCR can be a useful alternative to bacteriology to monitor VTEC infection in pig herds.

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